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Laboratory Update: Intrapartum Group B Strep testing by PCR

16 Sep 2018

Salem Health Laboratory now offers intrapartum screening for GBS



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As of July 2018, the Joint Commission added new requirements for pregnant women, specifically in labor and delivery.

To prevent neonatal infection during delivery, pre-assessing pregnant women’s status for HIV, syphilis, hepatitis B, and group B strep (GBS) is needed. If prenatal care is unknown or inadequate, testing should occur at the time of admission to labor and delivery.

Differences in GBS testing are outlined below:

 

Antepartum, 35-37 weeks

Intrapartum, labor/delivery

Test Code

BSCRN, LAB70014

GBSI, LAB3965

Test Name

Group B Strep Screen, PCR

Intrapartum Gr B Strep Screen, PCR

Collection Swab

Eswab, Stores item# 1772

 

Copan swab, Stores item # 7647

 

Collection Site

Vaginal/Rectal swab

Vaginal/Rectal swab (before exam, lubricants interfere with testing)

Stability

<24 hours room temperature, 6 days refrigerated

<24 hours room temperature, 6 days refrigerated

Turnaround time

24-36 hours

Within 2 hours of collection

Performed

Daily 0500-2200 at Regional Lab

24/7 at Priority Lab


Current guidelines from the Centers for Disease Control and Prevention and American College of Obstetricians and Gynecologists recommend universal screening of all pregnant women at 35 to 37 weeks of gestation for vaginal-rectal GBS colonization. The guidelines recommend antibiotic prophylaxis of patients who are screen positive, have GBS bacteria during the pregnancy, history of prior affected newborn, are less than 37 weeks gestation or develop other risk factors such as prolonged ruptured membranes beyond 18 hours or fever greater than 100.4.

The Joint Commission now additionally requires women more than 37 weeks with unknown GBS status to receive intrapartum antibiotic prophylaxis unless the result of a rapid intrapartum nucleic acid amplification test is available and negative.

We now have this intrapartum test available at Salem Hospital with an expected two-hour turnaround for results. This test should be limited to select patients at least 37 weeks admitted with unknown GBS results who are not expected to deliver imminently. This intrapartum test should not replace routine GBS screening at 35 to 37 weeks gestation in the majority of patients. Patients who have a negative intrapartum NAAT for GBS — but later develop risk factors of ruptured membranes for more than 18 hours or fever greater than 100.4 — would then meet indications for antibiotic prophylaxis.

For any questions or concerns, please contact:
Renee Gerros, MT, Senior Specialist Microbiology, 503-814-1640
Cheryl Lugenbill, MD, OB Hospitalist Medical Director, 503-814-4578